Phosphatidylinositol 4,5-bisphosphate phosphodiesterase in higher plants.

نویسندگان

  • W C McMurray
  • R F Irvine
چکیده

A phospholipase C which hydrolyses phosphatidylinositol 4,5-bisphosphate to release inositol trisphosphate was detected in a sedimentable fraction from celery and from some other higher plants. The particulate enzyme also hydrolyses phosphatidylinositol, whereas the soluble phosphatidylinositol phosphodiesterase described previously [Irvine, Letcher & Dawson (1980) Biochem. J. 192, 279-283] acts only on phosphatidylinositol, and we were unable to detect activity of this soluble activity on phosphatidylinositol 4,5-bisphosphate. Activity of the particulate enzyme is markedly enhanced in the presence of deoxycholate, but not of other detergents; the particulate enzyme can also be solubilized by extraction with deoxycholate.

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عنوان ژورنال:
  • The Biochemical journal

دوره 249 3  شماره 

صفحات  -

تاریخ انتشار 1988